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Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens

Received: 15 July 2023     Accepted: 4 August 2023     Published: 22 August 2023
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Abstract

The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation.

Published in American Journal of Nano Research and Applications (Volume 11, Issue 2)
DOI 10.11648/j.nano.20231102.11
Page(s) 19-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Nanotechnology, Green Synthesis, Nanoparticles, Guava, Antibacterial Activity

References
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Cite This Article
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    Tasabeeh Omer Abdulazeez, Ayat Ahmed Alrasheid, Ahmed Saeed Kabbashi, Saad Mohammed Hussein Ayoub. (2023). Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. American Journal of Nano Research and Applications, 11(2), 19-25. https://doi.org/10.11648/j.nano.20231102.11

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    ACS Style

    Tasabeeh Omer Abdulazeez; Ayat Ahmed Alrasheid; Ahmed Saeed Kabbashi; Saad Mohammed Hussein Ayoub. Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. Am. J. Nano Res. Appl. 2023, 11(2), 19-25. doi: 10.11648/j.nano.20231102.11

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    AMA Style

    Tasabeeh Omer Abdulazeez, Ayat Ahmed Alrasheid, Ahmed Saeed Kabbashi, Saad Mohammed Hussein Ayoub. Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens. Am J Nano Res Appl. 2023;11(2):19-25. doi: 10.11648/j.nano.20231102.11

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  • @article{10.11648/j.nano.20231102.11,
      author = {Tasabeeh Omer Abdulazeez and Ayat Ahmed Alrasheid and Ahmed Saeed Kabbashi and Saad Mohammed Hussein Ayoub},
      title = {Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens},
      journal = {American Journal of Nano Research and Applications},
      volume = {11},
      number = {2},
      pages = {19-25},
      doi = {10.11648/j.nano.20231102.11},
      url = {https://doi.org/10.11648/j.nano.20231102.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20231102.11},
      abstract = {The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Green Synthesis of Silver Nanoparticles from Psidium guajava Leaf Extract: Characterization and Evaluation of Antibacterial Activity Against Common Pathogens
    AU  - Tasabeeh Omer Abdulazeez
    AU  - Ayat Ahmed Alrasheid
    AU  - Ahmed Saeed Kabbashi
    AU  - Saad Mohammed Hussein Ayoub
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    PY  - 2023
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    DO  - 10.11648/j.nano.20231102.11
    T2  - American Journal of Nano Research and Applications
    JF  - American Journal of Nano Research and Applications
    JO  - American Journal of Nano Research and Applications
    SP  - 19
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2575-3738
    UR  - https://doi.org/10.11648/j.nano.20231102.11
    AB  - The abundant bioresources found in nature, including plants are used in the green synthesis of Silver nanoparticles. It offers an improvement over chemical and physical processes since it is less expensive and more environmentally friendly. This study aimed to synthesize silver nanoparticle using green method, characterization of silver nanoparticle using analytical techniques and to evaluate the antibacterial activity of synthesized silver nanoparticle. Psidium guajava L. leaf was collected, authenticated and extracted by water. A 1mM solution of silver nitrate was prepared. Afterwards, separate quantities of 1, 2, 3, 4, and 5mL of the plant extract were added to 10mL of silver nitrate to facilitate the synthesis of silver nanoparticles (AgNPs). The synthesized AgNps were characterized using UV/VIS Spectrometry, SEM analysis and FTIR analysis, also the antibacterial activity of AgNP at different concentrations against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was evaluated using well diffusion method. The synthesized Silver nanoparticle were detected by UV/ Vis spectrophotometer with absorbance peak at 459 nm. SEM analysis showed that silver nanoparticles were crystalline aggregate in nature and the size of AgNP was estimated between 1 - 200 nm. The reduction of silver nanoparticles and presence of functional groups of plant extract was confirmed by FTIR analysis of silver nanoparticles. AgNPs synthesized from P. guajava leaf revealed antimicrobial activity against all selected bacterial species. The use of nanotechnology in therapy development is an incredibly exciting and rapidly advancing field, the potential benefits of these emerging technologies make them a highly promising avenue for future medical research and innovation.
    VL  - 11
    IS  - 2
    ER  - 

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Author Information
  • Pharmaceutical Technology Program, Graduate College, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Biomedical Sciences, Faculty of Pharmacy, Omer Al-Mukhtar University, Al-Bayda, Libya

  • Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

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